Table 1 Antibodies used for immunocytochemistry
Target antigen | Antibody species | Vendor | Clone/product number | Dilution |
|---|---|---|---|---|
Pecam-1 | Rabbit | Thermo Scientific | RB-10333 | 1:25 |
Glut-1 | Mouse | Thermo Scientific | SPM498 | 1:500 |
Claudin-5 | Mouse | Life Technologies | 4C3C2 | 1:200 |
ZO-1 | Mouse | Thermo Scientific | 1A12 | 1:100 |
VE-Cadherin | Mouse | Santa Cruz | F-8 | 1:100 |
Occludin | Mouse | Life Technologies | OC-3F10 | 1:50 |
P-glycoprotein | Mouse | Thermo Scientific | P170(F4) | 1:25 |
β-tubulin III | Rabbit | Sigma | T3952 | 1:500 |
GFAP | Rabbit | Dako | ZO334 | 1:500 |
NG2 | Mouse | Millipore | MAB2029 | 1:100 |
PDGFR-β | Rabbit | Cell Signaling Technology | 28E1 | 1:100 |