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Fig. 6 | Fluids and Barriers of the CNS

Fig. 6

From: Human ES-derived MSCs correct TNF-α-mediated alterations in a blood–brain barrier model

Fig. 6

Effects of hES-MSCs on TNF-α-induced changes in gene expression of pro-inflammatory chemokines. BMECs were plated on 24-well Transwell inserts, allowed to achieve confluence, and then (±) exposed to 10 ng/ml TNF-α added to both the bottom and top chamber for 24 h at 37 °C. After this time, hES-MSCs were added to either the bottom (B) or top (T) chamber and incubated for and additional 24 h. Relative quantification of CCL2 and CXCL12 mRNA was determined by qRT-RCR. Changes in chemokine expression following different treatments were reported as x-fold change of control value. Data are presented as mean ± SE. Each experiment consisted of 3 replicates (derived from a single preparation of BMECs) repeated 3 times (each time from a different BMEC preparation), for a total N = 9 samples per group. *p < 0.01 compared with the control group; #p < 0.01 compared with TNF-α treated group

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