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Table 1 Summary of TEER results from in vitro co-culture platforms

From: Role of iPSC-derived pericytes on barrier function of iPSC-derived brain microvascular endothelial cells in 2D and 3D

Platform PC location Condition dhPC:dhBMEC ratio TEER ± SEM (Ω cm2) Comments
2D transwell Basolateral PCs Unstressed ECs 0:1 3510 ± 500 TEER independent of dhPC concentration
1:50 3690 ± 540
1:13 3600 ± 350
1:5 3690 ± 470
Stressed ECs 0:1 1800 ± 350 Increase in TEER with increasing dhPC concentration
1:50 2860 ± 660
1:13 3280 ± 320
1:5 3810 ± 290
Apical PCs   0:1 2680 ± 130  
P-on-E 1:50 2300 ± 430 Adding dhPCs after dhBMEC decreased TEER
1:5 1210 ± 450
E-on-P 1:50 1480 ± 790 Seeding dhPCs before dhBMEC decreased TEER
1:5 200 ± 180
E + P 1:50 1490 ± 730 Co-seeding of dhPCs and dhBMEC decreased TEER
1:5 300 ± 270
2.5D gel transwell Embedded PCs   0:1 2550 ± 970 Embedding dhPCs in Col I gel did not affect TEER
  1:13 2410 ± 520
3D microvessels Vessel-lining PCs   1:13 N/A Incorporating dhPC did not affect permeability to LY (444 Da)