Table 1 Summary of TEER results from in vitro co-culture platforms

2D transwell

Basolateral PCs

Unstressed ECs

0:1

3510 ± 500

TEER independent of dhPC concentration

1:50

3690 ± 540

1:13

3600 ± 350

1:5

3690 ± 470

Stressed ECs

0:1

1800 ± 350

Increase in TEER with increasing dhPC concentration

1:50

2860 ± 660

1:13

3280 ± 320

1:5

3810 ± 290

Apical PCs

0:1

2680 ± 130

P-on-E

1:50

2300 ± 430

Adding dhPCs after dhBMEC decreased TEER

1:5

1210 ± 450

E-on-P

1:50

1480 ± 790

Seeding dhPCs before dhBMEC decreased TEER

1:5

200 ± 180

E + P

1:50

1490 ± 730

Co-seeding of dhPCs and dhBMEC decreased TEER

1:5

300 ± 270

2.5D gel transwell

Embedded PCs

0:1

2550 ± 970

Embedding dhPCs in Col I gel did not affect TEER

1:13

2410 ± 520

3D microvessels

Vessel-lining PCs

1:13

N/A

Incorporating dhPC did not affect permeability to LY (444 Da)