Fig. 6

TEER, dhPC position, and dhPC density for dhBMEC monolayers on collagen I gels formed with encapsulated dhPCs. a Schematic illustration showing the 2.5D gel/transwell device. Experiments were performed with dhBMEC monolayers formed on 600 µm thick collagen gels (2.5 mg mL⁻¹) with or without encapsulated dhPCs. b Time dependence of TEER values for dhBMECs on gels with and without dhPCs. TEER values have been normalized to the peak of the control (no dhPCs), such that each biological replicate of the control reaches a maximum relative TEER of 1.0 at its highest point. bc Peak TEER for dhBMECs on gels with and without dhPCs. In b and c data represent mean ± SEM for two biological replicates (differentiations) and at least two technical replicates (transwells). d, e Position of dhPCs in gels with or without a dhBMEC monolayer on day 2 (d) or day 7 (e). The distance is referenced to the bottom of the well. f dhPC density in gels with or without a dhBMEC monolayer at day 2 and day 7. Data in d–f represent mean ± SD. At least 26 cells were quantified per condition per time point pooled from two gel replicates. ***P < 0.001