Fig. 5

Confocal images of dhBMEC monolayer formation during 2D contact co-culture with dhPC. GFP-dhBMECs were seeded 4 h after RFP-dhPCs on collagen IV/fibronectin coated glass. a Images showing dhBMECs (green) and dhPCs (red) during the first 10 h after seeding dhBMECs. b 3D reconstruction after 10 h. c Confocal images 48 h after seeding dhBMECs, taken at the (i) upper and (ii) lower locations of the cell layer. d Epifluorescence imaging following seeding a mixture of dhPCs and dhBMECs after (i, ii) 2 days and (iii, iv) 7 days