Fig. 2

Barrier function of dhBMEC monolayers on transwells in non-contact co-culture with dhPCs. a Schematic illustration of indirect co-culture in a transwell device. b Time dependence of TEER values for dhBMEC monolayers with different dhPC concentrations in the basolateral chamber. TEER values were normalized to the peak value of the control (no dhPCs), such that each biological replicate of the control reaches a maximum relative TEER of 1.0 at its highest point. c Peak TEER for dhBMEC monolayers. Peak TEER represents the TEER on whichever day the average of the technical replicates for that condition yielded the maximum TEER value. d Time dependence of TEER values for stressed dhBMEC monolayers (no ROCK inhibitor (-RI) during subculture) with different dhPC concentrations in the basolateral chamber. e Peak TEER for stressed dhBMEC monolayers. Data represent mean ± SEM, *P < 0.05. All experiments were performed with three biological replicates (independent dhBMEC differentiations) and at least two technical replicates (transwell experiments for each differentiation)