Fig. 3

CSF tracer distribution around blood vessels in control and constriction animals at 10 min post-injection. Representative micrographs from control (A, C) and constriction (B, D) animals at 1 week (A, B) or 6 weeks (C, D) post-surgery, sacrificed 10 min after injection of tracer into the cisterna magna. Insets show tracer (OA-647) distribution in the central gray matter (E, G) and peripheral white matter (F, H) and co-localized to arterioles (arrows), capillaries (arrowheads) and venules (open arrows). Individual channels from insets E–H are also shown: OA-647 tracer (I), rat endothelial cell antigen (RECA, II) and smooth muscle actin (SMA, III). Scale bars are 500 µm (A–D) and 20 µm (E–H)