Fig. 2

Macroscopic distribution of CSF tracer in the spinal cord 1 and 6 weeks post-constriction surgery. Graphs show mean fluorescence in each spinal segment measured macroscopically from (a, b) 1 week post-surgery in control (n = 4) and extradural constriction (n = 6 per group) animals and (c, d) 6 weeks post-surgery in control (n = 3 per group) and extradural constriction (10 min: n = 5; 20 min: n = 6) animals. Results are shown as mean fluorescence ± SEM, given as arbitrary units (AU). a, c 10 min after cisterna magna injection of CSF tracer, OA-647 (two-way ANOVA, [a] p = 0.4, [c] p < 0.0001; Bonferroni’s multiple comparison test, *p < 0.05, **p < 0.01). b, d 20 min after cisterna magna injection of CSF tracer (two-way ANOVA, p < 0.0001 for both; Bonferroni’s multiple comparison test, ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05)