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Fig. 5 | Fluids and Barriers of the CNS

Fig. 5

From: Cerebral influx of Na+ and Cl as the osmotherapy-mediated rebound response in rats

Fig. 5

Osmotherapy does not alter the brain barrier permeability. Na+-fluorescein (green fluorescence) was injected into the blood circulation of rats prior to i.p. exposure of isosmolar NaCl (control) or hyperosmolar NaCl (osmotherapy). Naïve rats did not receive Na+-fluorescein and were euthanized immediately after anaesthesia induction. a, e Phase contrast images illustrate structures of the brain regions of interest in transmitted white light. Representative images of Na+-fluorescein in bd hippocampus, thalamus, neocortex, and the lateral ventricle (LV) and fh pineal gland (positive control) of naïve rats, control rats, and osmotherapy-treated rats, n = 3. Scale bar = 500 μm

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