Fig. 2From: Sex-specific differences in organic anion transporting polypeptide 1a4 (Oatp1a4) functional expression at the blood–brain barrier in Sprague–Dawley ratsExpression of Slco1a4 mRNA and Oatp1a4 protein in brain microvessels isolated from male and female Sprague–Dawley rats. a Slco1a4 mRNA is increased in microvessels isolated from female Sprague–Dawley rats as compared to age-matched males. Results are expressed as mean ± SD of three experiments with each group consisting of mRNA samples from six individual experimental animals, **p < 0.01. b Western blot showed a single Oatp1a4 band at 90 kDa and a single band associated with tubulin at 50 kDa. While tubulin expression is the same between sexes, females (F1–6) show an approximately 3.5-fold higher expression of Oatp1a4 as compared to male animals (M1–6). Results are expressed as mean ± SD of the ratio of Oatp1a4 protein expression to tubulin protein expression from six animals per treatment group. MWM molecular weight marker. *p < 0.05Back to article page