Table 1 Results of nimodipine treatment in hydrocephalic ferrets
From: Nimodipine treatment does not benefit juvenile ferrets with kaolin-induced hydrocephalus
Non-hydrocephalic controls | Vehicle treated hydrocephalus | Low dose nimodipine hydrocephalus | High dose nimodipine hydrocephalus | |
|---|---|---|---|---|
Sample size (initial intent to treat) | 11 | 5 | 5 | 5 |
Lateral ventricle area index (P16) | 0.037 ± 0.004 | 0.092 ± 0.022* | 0.079 ± 0.012* | 0.100 ± 0.023* |
Lateral ventricle area index (P29/pre-treatment) | 0.023 ± 0.003 | 0.153 ± 0.042* | 0.145 ± 0.037* | 0.184 ± 0.046* |
Body weight (g) (P29/pre-treat) | 136.8 ± 8.0 | 114.7 ± 9.6 | 122.3 ± 12.6 | 120.2 ± 9.9 |
Survivors after early mortality related to vehicle | 7 | 3 | 4 | 3 |
Lateral ventricle area index (P29-censored after exclusion of early deaths) | 0.023 ± 0.004 | 0.153 ± 0.042* | 0.123 ± 0.038* | 0.185 ± 0.083* |
Lateral ventricle area index (P52/post-treatment) | 0.027 ± 0.003 | 0.192 ± 0.067* | 0.238 ± 0.112 | 0.303 ± 0.122* |
Enlargement ventricles during treatment (%) | – | 25.2 ± 32.3 | 93.5 ± 36.0 | 63.7 ± 27.2 |
Body weight (g) (P29-censored after exclusion of early deaths) | 125.4 ± 8.5 | 114.7 ± 9.6 | 120.8 ± 11.2 | 128.0 ± 9.2 |
Body weight (g) (P52/post-treat) | 299.9 ± 13.7# | 288.5 ± 42.5# | 272.7 ± 58.7# | 310.5 ± 4.5# |
Body weight increase during treatment (%) | 139.2 ± 22.7 | 151.5 ± 35.5 | 125.7 ± 43.6 | 142.6 ± 41.8 |
Rearing activity (beam breaks per 3 min) (P48–50) | 112 ± 15 | 88 ± 19 | 29 ± 18* | 84 ± 53 |
Ambulatory activity (beam breaks per 3 min) (P48–50) | 700 ± 74 | 604 ± 88 | 1143 ± 615 | 971 ± 225 |
Total activity (beam breaks per 3 min) (P48–50) | 930 ± 68 | 783 ± 110 | 1353 ± 670 | 1153 ± 230 |
Open field—number cells entered (per 3 min) (P48–50) | 122 ± 14 | 126 ± 12 | 259 ± 146 | 129 ± 17 |
Open field—percent cells entered (per 3 min) (P48–50) | 64 ± 6 | 71 ± 4 | 51 ± 9 | 59 ± 10 |
Open field—distance traveled (m per 3 min) (P48–50) | 7.83 ± 0.96 | 7.77 ± 0.79 | 18.27 ± 12.25 | 7.84 ± 1.21 |
Diminished vision/blind (P48–50) | 0/0 | 0/1 | 0/0 | 1/2 |
Medial corpus callosum thickness (μm) | 544 ± 44 | 288 ± 74* | 198 ± 29* | 291 ± 79* |
Lateral corpus callosum thickness (μm) | 397 ± 32 | 272 ± 100 | 204 ± 23* | 269 ± 73 |
Ki-67 positive cells ratio in SVZ (%) | 16.1 ± 2.4 | 7.0 ± 1.9* | 15.5 ± 3.5 | 7.1 ± 2.3* |
GFAP content (ELISA) frontal cerebrum (μg GFAP/g protein) | 0.446 ± 0.088 | 0.640 ± 0.361 | 0.373 ± 0.037 | 0.572 ± 0.178 |