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Table 1 Results of nimodipine treatment in hydrocephalic ferrets

From: Nimodipine treatment does not benefit juvenile ferrets with kaolin-induced hydrocephalus

  Non-hydrocephalic controls Vehicle treated hydrocephalus Low dose nimodipine hydrocephalus High dose nimodipine hydrocephalus
Sample size (initial intent to treat) 11 5 5 5
Lateral ventricle area index (P16) 0.037 ± 0.004 0.092 ± 0.022* 0.079 ± 0.012* 0.100 ± 0.023*
Lateral ventricle area index (P29/pre-treatment) 0.023 ± 0.003 0.153 ± 0.042* 0.145 ± 0.037* 0.184 ± 0.046*
Body weight (g) (P29/pre-treat) 136.8 ± 8.0 114.7 ± 9.6 122.3 ± 12.6 120.2 ± 9.9
Survivors after early mortality related to vehicle 7 3 4 3
Lateral ventricle area index (P29-censored after exclusion of early deaths) 0.023 ± 0.004 0.153 ± 0.042* 0.123 ± 0.038* 0.185 ± 0.083*
Lateral ventricle area index (P52/post-treatment) 0.027 ± 0.003 0.192 ± 0.067* 0.238 ± 0.112 0.303 ± 0.122*
Enlargement ventricles during treatment (%) 25.2 ± 32.3 93.5 ± 36.0 63.7 ± 27.2
Body weight (g) (P29-censored after exclusion of early deaths) 125.4 ± 8.5 114.7 ± 9.6 120.8 ± 11.2 128.0 ± 9.2
Body weight (g) (P52/post-treat) 299.9 ± 13.7# 288.5 ± 42.5# 272.7 ± 58.7# 310.5 ± 4.5#
Body weight increase during treatment (%) 139.2 ± 22.7 151.5 ± 35.5 125.7 ± 43.6 142.6 ± 41.8
Rearing activity (beam breaks per 3 min) (P48–50) 112 ± 15 88 ± 19 29 ± 18* 84 ± 53
Ambulatory activity (beam breaks per 3 min) (P48–50) 700 ± 74 604 ± 88 1143 ± 615 971 ± 225
Total activity (beam breaks per 3 min) (P48–50) 930 ± 68 783 ± 110 1353 ± 670 1153 ± 230
Open field—number cells entered (per 3 min) (P48–50) 122 ± 14 126 ± 12 259 ± 146 129 ± 17
Open field—percent cells entered (per 3 min) (P48–50) 64 ± 6 71 ± 4 51 ± 9 59 ± 10
Open field—distance traveled (m per 3 min) (P48–50) 7.83 ± 0.96 7.77 ± 0.79 18.27 ± 12.25 7.84 ± 1.21
Diminished vision/blind (P48–50) 0/0 0/1 0/0 1/2
Medial corpus callosum thickness (μm) 544 ± 44 288 ± 74* 198 ± 29* 291 ± 79*
Lateral corpus callosum thickness (μm) 397 ± 32 272 ± 100 204 ± 23* 269 ± 73
Ki-67 positive cells ratio in SVZ (%) 16.1 ± 2.4 7.0 ± 1.9* 15.5 ± 3.5 7.1 ± 2.3*
GFAP content (ELISA) frontal cerebrum (μg GFAP/g protein) 0.446 ± 0.088 0.640 ± 0.361 0.373 ± 0.037 0.572 ± 0.178
  1. Data are presented as mean ± standard error of the mean (SEM)
  2. Behavior and ventricle size are specified at by postnatal day (P) age in days. Histological and glial fibrillary acidic protein (GFAP) data are at P52
  3. *p < 0.05 control vs. hydrocephalic, t tests or ANOVA
  4. #p < 0.05 P29 (pre-treat) vs. P52 (post-treat), t tests