Fig. 4

Functional microvessels formed from dhBMECs. a Schematic of microvessel construction. b BC1 GFP—dhBMEC (GFP) growth in genipin cross-linked 7 mg mL⁻¹ collagen gel, coated with collagen IV and fibronectin, with RI, cAMP, Ro-20-1724, and 70 kDa dextran. c Confocal image of a dhBMEC microvessel, cross section, and projected image at the bottom of the microvessel. ZO-1 (red), DAPI (blue). d Sequence of fluorescence images during a Lucifer yellow permeability experiment. e Normalized fluorescence intensity versus time following Lucifer yellow introduction into the microvessel