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Fig. 2 | Fluids and Barriers of the CNS

Fig. 2

From: Functional brain-specific microvessels from iPSC-derived human brain microvascular endothelial cells: the role of matrix composition on monolayer formation

Fig. 2

Barrier function of dhBMEC monolayers. a Schematic illustration of transwell assay for TEER measurements for dhBMEC monolayers on coated membranes or collagen I gels and membrane coatings. b Peak TEER on membranes coated with basement membrane proteins and heparan sulfate proteoglycans that resulted in monolayer formation coverage where the control is cells directly subcultured onto fn + cnIV coated membranes, compared to cells subcultured on plastic tissue culture plates coated with collagen IV and fibronectin before plating onto coated transwells. c Peak TEER taken on membranes coated with collagen gels and basement membrane coating mixtures. d Peak TEER on transwells with collagen gels coated with fibronectin and collagen IV, and different combinations of ROCK inhibitor Y27632, cAMP, and genipin treatment. Bars represent mean ± standard error, * represents p < 0.05, ** represents p < 0.01, *** represents p < 0.001

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