Fig. 3

Time-, temperature-, and concentration-dependence of [³H]p-TA uptake by TR-CSFB3 cells. a [³H]p-TA uptake (0.15 μCi/well, 19 nM) was measured at 37 °C (open circles) and 4 °C (closed square) for indicated times. The solid line was obtained by MULTI analysis. Each point represents the mean ± S.E.M. (n = 3). **p < 0.01, significantly different from [³H]p-TA uptake at 37 °C for 2 min. b p-TA uptake, over the concentration range 20 nM–60 mM, was measured at 37 °C for 2 min. The data were subjected to Eadie–Scatchard analysis in addition to Michaelis–Menten kinetics (inset). The solid, dashed, and dotted lines, which were fitted by the MULTI program, represent overall, saturable, and non-saturable transport, respectively. Each point represents the mean ± S.E.M. (n = 3)