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Fig. 2 | Fluids and Barriers of the CNS

Fig. 2

From: LDL receptor blockade reduces mortality in a mouse model of ischaemic stroke without improving tissue-type plasminogen activator-induced brain haemorrhage: towards pre-clinical simulation of symptomatic ICH

Fig. 2

Receptor-associated protein (RAP) does not significantly attenuate rt-PA-induced BBB disruption and formation of intracerebral haemorrhage. Receptor-associated protein (2 mg/kg) was co-administered with rt-PA (10 mg/kg) post 4 h middle cerebral artery occlusion (MCAo) and blood components were measured in the perfused brain 20 h later. a Albumin values in the ipsilateral hemisphere (after subtraction of the contralateral values to correct for perfusion efficiency) and b fold analysis of brain albumin (ipsilateral above contralateral, accounting for each animal’s own baseline). Administration of RAP together with rt-PA does not reduce albumin levels compared to rt-PA alone (a) but a trend for reduced albumin with RAP is apparent by fold (b). c, d Brain haemoglobin is increased with rt-PA compared to vehicle both in the raw analysis (ipsilateral minus contralateral; c) or the relative analysis (fold; ipsilateral above contralateral; d). While a trend emerges, addition of RAP with rt-PA does not significantly attenuate blood levels in the brain. e Representative images of brain tissue 24 h after rt-PA or rt-PA + RAP treatment post 4 h MCAo. Significant intraparenchymal hematomas can still be observed after RAP treatment. Data is shown as individual animals with mean ± SEM. n = 11 for vehicle, 10 for rt-PA, 8 for rt-PA + RAP and 7 for RAP. One-way ANOVA with Sidak post hoc analysis of selected groups. Outliers are denoted in black symbols and excluded from the analysis

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