Fig. 3

BMECs differentiated using UM and E6 medium demonstrate equivalent maximum TEER and stability. For each medium, three filters were seeded with BMECs, and TEER was measured in three different positions for each filter. Each plot is the result of one biological replicate (n = 1) with each daily TEER measurement the result of a technical n = 9. Values are reported as mean ± standard deviation of these collective measurements. a BMECs differentiated using E6 medium demonstrated maximum TEER values of at least 2500 Ω × cm² for a minimum of 11 days in three biological replicates (i–iii). TEER was measured approximately every 24 h for the duration of the experiment. b BMECs differentiated using UM demonstrated maximum TEER values of at least 2500 Ω × cm² for a minimum of 12 days in three biological replicates (i–iii). TEER was measured approximately every 24 h for the duration of the experiment