Papp A→B (cm s−1) | N | Papp B→A (cm s−1) | N | Efflux ratio | |
---|---|---|---|---|---|
100 µM 2-PAM | |||||
MDCKII | 2.99 ± 1.12 × 10−6 | 11 | 2.48 ± 1.30 × 10−6 | 8 | 0.82 |
MDCKII-MDR1 | 3.01 ± 1.27 × 10−6 | 8 | 2.51 ± 1.08 × 10−6 | 7 | 0.83 |
MDCKII-FLuc-ABCG2 | 0.76 ± 0.05 × 10−6 | 7 | 0.98 ± 0.40 × 10−6 | 7 | 1.30 |
BC1-hBMECs | 1.12 ± 0.80 × 10−6 (n = 5) | 18 | 0.49 ± 0.16 × 10−6 (n = 3) | 12 | 0.84 |
10 µM 2-PAM | |||||
MDCKII | 1.62 ± 0.21 × 10−6 | 6 | 1.29 ± 1.76 × 10−6 | 7 | 0.80 |
MDCKII-MDR1 | 2.03 ± 0.14 × 10−6 | 8 | 1.18 ± 0.45 × 10−6 | 8 | 0.58 |
MDCKII-FLuc-ABCG2 | 0.83 ± 0.35 × 10−6 | 7 | 0.99 ± 0.62 × 10−6 | 7 | 1.18 |
50 µM R123 | |||||
MDCKII | 0.30 ± 0.20 × 10−6 | 6 | 3.18 ± 0.60 × 10−6 | 5 | 10.7 |
MDCKII-MDR1 | 0.21 ± 0.21 × 10−6 | 12 | 4.36 ± 0.41 × 10−6 | 11 | 20.3 |
100 µM LY | |||||
MDCKII | 0.71 ± 0.34 × 10−6 | 7 | |||
MDCKII-MDR1 | 0.38 ± 0.20 × 10−6 | 7 | |||
MDCKII-FLuc-ABCG2 | 0.46 ± 0.21 × 10−6 | 8 | |||
2-PAM atropine | |||||
MDCKII | 2.54 ± 0.33 × 10−6 | 7 |