Fig. 2
From: MicroRNA-155 contributes to shear-resistant leukocyte adhesion to human brain endothelium in vitro
miR-155 modulates VCAM1 and ICAM1 expression on brain endothelial hCMEC/D3 cells. hCMEC/D3 cell monolayers were transfected with control siRNA (a, b) or scrambled Pre-miR and Pre-miR-155 (c, e) or Anti-miR and Anti-miR-155 (d, f) followed by treatment with a combination of cytokines (TNFα + IFNγ) at 1 ng/ml for 24 h or left unstimulated. a, b Number of shear-resistant firmly adhered Jurkat and THP-1 cells to siVCAM1-hCMEC/D3 monolayer per FOV (640 × 480 μm). c–f VCAM1 and ICAM1 expression levels were quantified by ELISA. Experiments were carried out three and four times with three replicates each. Data are mean ± SEM. Statistical analysis was performed using paired Student’s t test (*,# P < 0.05, ***,### P < 0.001, #compared to unstimulated)