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Fig. 3 | Fluids and Barriers of the CNS

Fig. 3

From: Modeling immune functions of the mouse blood–cerebrospinal fluid barrier in vitro: primary rather than immortalized mouse choroid plexus epithelial cells are suited to study immune cell migration across this brain barrier

Fig. 3

Morphology, expression of transthyretin and propagation of ECPC4 cells. Representative phase contrast pictures of ECPC4 cells from passage 41 on d1 (a) and d4 (b) after sub-cultured in a 1:4 ratio as described by the distributer. The cells did not show typical epithelial morphology (a, b) and grew in overlapping layers (b). Scale bars 50 μm. c Immunofluorescence staining for the CPEC-specific marker transthyretin (TTR) is shown in pmCPECs, Immortomouse® CPECs from passage 6, grown for 7 days at non-permissive conditions and for the ECPC4 cells. Scale bars a, b and ECPC4 cells = 50 μm; pmCPECs and Immortomouse® CPECs = 100 μm

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