Table 1 Initial seeding density effects on hPSC-derived brain microvascular endothelial cells
Cell line | Days in mTeSR1 | Density at D0 (cells/cm2) | RA conc. (μM) | % VE-cadherin+a | Max. TEER (Ω × cm2)b |
|---|---|---|---|---|---|
IMR90-4 | 3 | 30,000 | 10 | 67 ± 8 | 2,300 ± 290 |
3 | 100,000 | 10 | 94 ± 1 | 2,310 ± 100 | |
3 | 30,000 | 0 | ND | 310 ± 50 | |
1 | 30,000 | 10 | 39 ± 23 | 1,860 ± 470 | |
DF19-9-11 | 3 | 10,000 | 10 | 72 ± 10 | 960 ± 40 |
3 | 30,000 | 10 | 76 ± 7 | 670 ± 40 | |
3 | 100,000 | 10 | 92 ± 6 | 450 ± 100 | |
H9 | 3 | 30,000 | 10 | 69 ± 15 | 2,190 ± 500 |
3 | 100,000 | 10 | 73 ± 1 | 375 ± 410 |