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Figure 3 | Fluids and Barriers of the CNS

Figure 3

From: In vitroeffects of fetal rat cerebrospinal fluid on viability and neuronal differentiation of PC12 cells

Figure 3

PC12 cells after 7 days in culture photographed with phase-contrast optics. A: control culture, B: culture with b-FGF, C: culture with added CSF-E17, D: with CSF-E18, E: with CSF-E19 and F: with CSF-E20. PC12 cells cultured with ECSF from E17 and E19, and with b-FGF showed neurite outgrowth and morphological differentiation, whereas cells cultured with E-CSF from E18, E20, and the control group (A) did not show any significant differentiation.

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