Figure 2From: In vitroeffects of fetal rat cerebrospinal fluid on viability and neuronal differentiation of PC12 cellsSurvival and/or proliferation of PC12 cells after culture with fetal rat CSF. Reduction of MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) measured colourimetrically by the absorbance of formazan product. Cells were treated with CSF at different gestational ages (E17-E20) and measured after 7 days in culture. Results are expressed as a percentage of control levels (cultures without added CSF). All cultures with added CSF had higher viability and this was significant with E18 CSF. Data are mean ± SEM, n =9, *; p <0.05 compared to controls.Back to article page