Figure 4

Co-localisation of fluorescein-labelled dextran with ED1-positive perivascular macrophages. Ten minutes after CED there was widespread co-localisation of fluorescein-labelled dextran (green) with perivascular macrophages immunostained for ED1 (red). Representative images from animals infused with 10 and 150 kDa dextran are shown in A-F. Scale bar = 100 μm. Stereological cell counts demonstrated significantly higher co-localisation of 4, 10 and 20 kDa dextrans than 150 kDa dextran, 10 min after CED. For 4 and 20 kDa dextran, the increase in co-localisation was highly significant (* p < 0.01). However by 3 h there were no significant differences in uptake (figure G). Each histogram bar represents mean cell counts from 3 striata +/- S.D.