Figure 4

In vitro blood–brain barrier permeability investigation. The relative permeability of endothelial monolayers to Lucifer Yellow was assessed after 16 days of culture with different cell types. The monolayers were first cultured with glial cells for 12 days and then cultured for 4 days (i) alone ("soloculture"), (ii) with glial cells (" + glial cells"), and (iii) with porcine pericytes from the different genotypes (" + NN pericytes", " + Nn pericytes" and + "nn pericytes"), according to the protocol described in Figure 3A. The results represent the mean ± s.e.m of 3 independent experiments. For statistical purposes, a one-way analysis of variance was followed by a Tukey test (p < 0.05) with n = 6. The reference permeability coefficient value was 0.28 × 10^-3 cm/min, corresponding to the coculture condition (+ glial cells). N.S.: non-significant.