Figure 8

Cell-specific regulation of LC3-II expression in barrier associated cells. Representative Western Blots of LC3-I (A) and LC3-II (B) expression in ECs, ACs and PCs are shown in lower panels, upper panels represent graphs of densitometric analysis. Cells were exposed to NX, HX and AX in presence and absence of glucose for 24 h and 48 h. N = 4. *P < 0.05, **P < 0.01, ***P < 0.001; 1-way ANOVA compared to normoxia + glucose. ^§ P < 0.05, ^§§ P < 0.01, ^§§§ P < 0.001; 1-way ANOVA compared to normoxia-glucose. ^# P < 0.05, ^## P < 0.01, ^### P < 0.001; 2-way ANOVA comparing + to - glucose treatment. EC cell line: RBE4, AC: astrocyte, PC: pericyte, NX: normoxia, HX: hypoxia, AX: near anoxia, +Glc: with glucose, -Glc: without glucose.