Figure 7
Prolonged and severe hypoxic/ischemic treatment decreases Bax and Beclin-1 expression. Densitometric quantification of Bax (A) and Beclin-1 (B) protein expression (upper panel) and representative Western Blots (lower panels) after 24 h and 48 h of O2 deprivation and ischemic treatment in EC cell line, ACs and PCs. N = 4. *P < 0.05, **P < 0.01, ***P < 0.001; 1-way ANOVA compared to normoxia + glucose. § P < 0.05, §§ P < 0.01, §§§ P < 0.001; 1-way ANOVA compared to normoxia-glucose. # P < 0.05, ## P < 0.01, ### P < 0.001; 2-way ANOVA comparing + to - glucose treatment. EC cell line: RBE4, AC: astrocyte, PC: pericyte, NX: normoxia, HX: hypoxia, AX: near anoxia, +Glc: with glucose, -Glc: without glucose.