Figure 6

Differential regulation of BNIP3 expression in barrier associated cells. BNIP3 mRNA and protein expression was measured after 24 h and 48 h exposure to HX, AX and OGD and compared to NX. (A) Table of basal normoxic expression of BNIP3 showing threshold cycles (CT) ± standard deviation (upper panel). Lower panels BNIP3 mRNA expression after 24 h (left) and 48 h (right) of treatment. (B) Densitometric quantification of BNIP3 protein expression (upper panel) and representative Western blots (lower panels). N = 4. *P < 0.05, **P < 0.01, ***P < 0.001; 1-way ANOVA compared to normoxia + glucose. ^§ P < 0.05, ^§§ P < 0.01, ^§§§ P < 0.001; 1-way ANOVA compared to normoxia-glucose. ^# P < 0.05, ^## P < 0.01, ^### P < 0.001; 2-way ANOVA comparing + to - glucose treatment. EC cell line: RBE4, AC: astrocyte, PC: pericyte, NX: normoxia, HX: hypoxia, AX: near anoxia, +Glc: with glucose, -Glc: without glucose.