Figure 4

Adaptation of proliferation by barrier associated cells during hypoxic and ischemic insults. Proliferation of primary ECs, ACs and PCs was analyzed using BrdU incorporation assay after exposure to NX, HX and AX in presence (solid bars) and absence (hatched bars) of glucose for 24 h (A) and 48 h (B). Data values were normalized to normoxic baseline (NX + Glc). N = 3-5. *P < 0.05, **P < 0.01, ***P < 0.001; 1-way ANOVA compared to normoxia + glucose. ^§§§ P < 0.001; 1-way ANOVA compared to normoxia-glucose. ^# P < 0.05, ^## P < 0.01, ^### P < 0.001; 2-way ANOVA comparing + to - glucose. EC: primary endothelial cells, AC: primary astrocyte, PC: primary pericyte, NX: normoxia, HX: hypoxia, AX: near anoxia, +Glc: with glucose, -Glc: without glucose.