Figure 3

O ₂ deprivation and ischemia induce actin cytoskeleton rearrangements in barrier associated cells. F-actin fibers stained with phalloidin (white) and cell nuclei with DAPI (blue). (A) Monolayers of EC cell line exposed to normoxia, hypoxia and near anoxia for 6 and 24 h. (B) F-actin staining of primary endothelial cell (upper panel), astrocytes (middle panel) and pericytes (lower panel) exposed to normoxia and near anoxia in presence or absence of glucose for 24 h. White arrow shows stress fiber formation; red arrows indicate inter-endothelial gap formation. Asterisk highlights breakdown of F-actin resulting in punctuate staining.