Figure 2
Differential regulation of HIF-1 target genes in barrier associated cells by quantitative PCR. (A) Table of basal normoxic expression of the analyzed genes showing threshold cycles (CT) ± standard deviation. mRNA expression profiles of PHD2 (B), VEGF (C), GLUT-1 (D) and MMP-9 (E) after 24 h (left panel) and 48 h (right panel) of O2 deprivation (solid bars) and oxygen glucose deprivation (hatched bars). N = 3-4. *P < 0.05, **P < 0.01, ***P < 0.001; 1-way ANOVA compared to normoxic baseline. § P < 0.05, §§ P < 0.01, §§§ P < 0.001; 1-way ANOVA compared to normoxia-glucose. # P < 0.05, ## P < 0.01, ### P < 0.001; 2-way ANOVA comparing with (+) to without (-) glucose treatments. EC cell line: RBE4, AC: astrocyte, PC: pericyte, NX: normoxia, HX: hypoxia, AX: near anoxia, +Glc: with glucose, -Glc: without glucose, PHD2: prolyl hydroxylase 2, VEGF: vascular endothelial growth factor, GLUT-1: glucose transporter 1, MMP-9: matrix metalloproteinase 9.