Figure 1

HIF-1α induction profiles in barrier associated cells. Western blot analysis of HIF-1α protein expression in EC cell line, PCs and ACs exposed to HX (1% O₂) and AX (0.2% O₂) in presence or absence of glucose for up to 24 h. (A) Densitometric quantification of HIF-1α protein expression in EC (upper left panel), PC (upper right panel) and AC (lower left panel). N = 3; *P < 0.05, **P < 0.01, ***P < 0.001; 1-way ANOVA compared to normoxic baseline. Representative comparative Western blots of HIF-1α expression in different cell types and their densitometric quantifications after exposure to hypoxia (B) and near anoxia (C). N = 3. ^# P < 0.05, ^## P < 0.01, ^### P < 0.001; 2-way ANOVA compared to EC cell line. EC cell line: RBE4, AC: astrocyte, PC: pericyte, HX: hypoxia, AX: near anoxia, +Glc: with glucose, -Glc: without glucose.