Figure 13
From: Impedance-based cell monitoring: barrier properties and beyond
Schematic illustration of the experimental setup to determine the impact of different endogenous extracellular matrices (ECM) on the integrity of brain capillary endothelial cells (PBCEC) using ECIS technique. (A) Establishment of endogenous extracellular matrices derived from astrocytes, pericytes, aorta (PAEC) and cerebral endothelial cells (PBCEC) on the ECIS electrode surfaces (1st step). Seeding of PBCEC on ECIS electrodes that had been pre-coated with endogenous extracellular matrices. Monitoring the progression of the barrier formation of PBCEC on different matrices (2nd step) by ECIS technique. (B, C) Analysis of the impact of extracellular matrices on the barrier integrity on PBCECs. Time course of the resistance measured at a sampling frequency of 400 Hz. (B) Comparison between astrocyte, pericyte and PCBEC derived endogenous extracellular (as control) matrix on the integrity of PBCEC. (C) Represents the relation between aorta derived matrices to endothelial derived matrices (control). Each data point represents the mean ± SD From [33] with permission.