In vivo hyperspectral fluorescence image of control mouse. (a) Ventral view of the head and neck 6 h after QD application onto the dura mater covering the cisterna magna. No QD signal (red) is visible. Skin/fur autofluoresence (green), autofluorescence from food (blue). (b) Spectral emission profile (grey dotted line) of the region indicated by the arrow in (a) shows that signal in the submandibular region matched the profile for skin/fur autofluorescence (green line) and is distinct from QD signal (red line) and that of autofluorescence from food (blue line). Scale = 1 cm.