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Table 1 Summary of maximal TEER values recorded real-time by the CellZscope system for hCMEC/D3, hBMEC, and TY10 mono-cultures

From: Comparative study of four immortalized human brain capillary endothelial cell lines, hCMEC/D3, hBMEC, TY10, and BB19, and optimization of culture conditions, for an in vitro blood–brain barrier model for drug permeability studies

24-well tissue culture insert coated with collagen type I hCMEC/D3 hBMEC TY10
Time (d) Mean max. TEER ± S.E.M. (Ωcm2) Time (d) Mean max. TEER ± S.E.M. (Ωcm2) Time (d) Mean max. TEER ± S.E.M. (Ωcm2)
Corning® (translucent PC, 0.4 μm, 1.0 × 108 pores/cm2, 0.33 cm2) 4.75 6.72 (± 0.113) 2.46 2.79 (± 0.410) 2.37 4.56 (± 1.06)
Corning® (translucent PC, 3.0 μm, 2.0 × 106 pores/cm2, 0.33 cm2) 5.07 8.56 (± 0.217) 5.19 #9.09 (± 0.103) 7.73** #11.2 (± 0.904)
Corning® (transparent PES, 0.4 μm, 4.0 × 106 pores/cm2, 0.33 cm2) 1.97* 7.04 (± 0.231) 2.79* 17.7 (± 0.152) 1.96 6.96 (± 0.199)
Corning® (transparent PES, 3.0 μm, 2.0 × 106 pores/cm2, 0.33 cm2) 3.38 9.44 (± 0.0877) 2.81 6.24 (± 0.0805) 2.37 7.94 (± 0.110)
Greiner Bio-one® (transparent PET, 0.4 μm, 2.0 × 106 pores/cm2, 0.336 cm2) 2.47 6.48 (± 0.125) 1.85 #7.27 (± 2.36) 1.45* 9.37 (± 0.690)
Greiner Bio-one® (translucent PET, 0.4 μm, 1.0 × 108 pores/cm2, 0.336 cm2) 2.34 5.18 (± 0.222) 5.13 7.42 (± 0.288) 1.66* 12.4 (± 2.11)
Greiner Bio-one® (transparent PET, 3.0 μm, 0.6 × 106 pores/cm2, 0.336 cm2) 2.34 11.9 (± 0.118) 2.75 28.4 (± 2.47) 1.15* 13.0 (± 0.0151)
Greiner Bio-one® (translucent PET, 3.0 μm, 2.0 × 106 pores/cm2, 0.336 cm2) 2.34 9.39 (± 0.166) 2.49 5.85 (± 0.357) 1.32* 7.26 (± 0.0949)
Millipore® (translucent PET, 0.4 μm, 1.0 × 108 pores/cm2, 0.33 cm2) 2.34 5.35 (± 0.312) 2.49 2.92 (± 0.164) 1.37* 5.98 (± 0.200)
Millipore® (translucent PET, 3.0 μm, 2.0 × 106 pores/cm2, 0.33 cm2) 2.34 10.2 (± 0.0304) 2.53 15.0 (± 0.348) 2.37 11.2 (± 0.806)
BD Falcon® (transparent PET, 0.4 μm, 2.0 × 106 pores/cm2, 0.3 cm2) 2.34 5.09 (± 0.133) 3.34 8.40 (± 0.524) 3.11 4.72 (± 0.00525)
BD Falcon® (transparent PET, 3.0 μm, 0.8 × 106 pores/cm2, 0.3 cm2) 2.34 8.75 (± 0.180) 3.21 24.1 (± 0.0595) 1.54* 6.19 (± 0.429)
  1. All tested 24-well tissue culture inserts were coated with collagen type I. The cell seeding density varied between 3.0 × 104 and 15 × 104 cells/cm2 for hCMEC/D3, between 4.5 × 104 and 17 × 104 cells/cm2 for hBMEC, and between 1.5 × 104 and 15 × 104 cells/cm2 for TY10 (n = 2–3).
  2. #CCL outside the acceptable range of 0.5-5.0 μF/cm2 almost during the whole experiment, indicating that cells were not confluent; PC: polycarbonate; PES: polyester; PET: polyethylene terephthalate; *before first change of medium; **after third change of medium.