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Table 1 Summary of maximal TEER values recorded real-time by the CellZscope system for hCMEC/D3, hBMEC, and TY10 mono-cultures

From: Comparative study of four immortalized human brain capillary endothelial cell lines, hCMEC/D3, hBMEC, TY10, and BB19, and optimization of culture conditions, for an in vitro blood–brain barrier model for drug permeability studies

24-well tissue culture insert coated with collagen type I

hCMEC/D3

hBMEC

TY10

Time (d)

Mean max. TEER ± S.E.M. (Ωcm2)

Time (d)

Mean max. TEER ± S.E.M. (Ωcm2)

Time (d)

Mean max. TEER ± S.E.M. (Ωcm2)

Corning® (translucent PC, 0.4 μm, 1.0 × 108 pores/cm2, 0.33 cm2)

4.75

6.72 (± 0.113)

2.46

2.79 (± 0.410)

2.37

4.56 (± 1.06)

Corning® (translucent PC, 3.0 μm, 2.0 × 106 pores/cm2, 0.33 cm2)

5.07

8.56 (± 0.217)

5.19

#9.09 (± 0.103)

7.73**

#11.2 (± 0.904)

Corning® (transparent PES, 0.4 μm, 4.0 × 106 pores/cm2, 0.33 cm2)

1.97*

7.04 (± 0.231)

2.79*

17.7 (± 0.152)

1.96

6.96 (± 0.199)

Corning® (transparent PES, 3.0 μm, 2.0 × 106 pores/cm2, 0.33 cm2)

3.38

9.44 (± 0.0877)

2.81

6.24 (± 0.0805)

2.37

7.94 (± 0.110)

Greiner Bio-one® (transparent PET, 0.4 μm, 2.0 × 106 pores/cm2, 0.336 cm2)

2.47

6.48 (± 0.125)

1.85

#7.27 (± 2.36)

1.45*

9.37 (± 0.690)

Greiner Bio-one® (translucent PET, 0.4 μm, 1.0 × 108 pores/cm2, 0.336 cm2)

2.34

5.18 (± 0.222)

5.13

7.42 (± 0.288)

1.66*

12.4 (± 2.11)

Greiner Bio-one® (transparent PET, 3.0 μm, 0.6 × 106 pores/cm2, 0.336 cm2)

2.34

11.9 (± 0.118)

2.75

28.4 (± 2.47)

1.15*

13.0 (± 0.0151)

Greiner Bio-one® (translucent PET, 3.0 μm, 2.0 × 106 pores/cm2, 0.336 cm2)

2.34

9.39 (± 0.166)

2.49

5.85 (± 0.357)

1.32*

7.26 (± 0.0949)

Millipore® (translucent PET, 0.4 μm, 1.0 × 108 pores/cm2, 0.33 cm2)

2.34

5.35 (± 0.312)

2.49

2.92 (± 0.164)

1.37*

5.98 (± 0.200)

Millipore® (translucent PET, 3.0 μm, 2.0 × 106 pores/cm2, 0.33 cm2)

2.34

10.2 (± 0.0304)

2.53

15.0 (± 0.348)

2.37

11.2 (± 0.806)

BD Falcon® (transparent PET, 0.4 μm, 2.0 × 106 pores/cm2, 0.3 cm2)

2.34

5.09 (± 0.133)

3.34

8.40 (± 0.524)

3.11

4.72 (± 0.00525)

BD Falcon® (transparent PET, 3.0 μm, 0.8 × 106 pores/cm2, 0.3 cm2)

2.34

8.75 (± 0.180)

3.21

24.1 (± 0.0595)

1.54*

6.19 (± 0.429)

  1. All tested 24-well tissue culture inserts were coated with collagen type I. The cell seeding density varied between 3.0 × 104 and 15 × 104 cells/cm2 for hCMEC/D3, between 4.5 × 104 and 17 × 104 cells/cm2 for hBMEC, and between 1.5 × 104 and 15 × 104 cells/cm2 for TY10 (n = 2–3).
  2. #CCL outside the acceptable range of 0.5-5.0 μF/cm2 almost during the whole experiment, indicating that cells were not confluent; PC: polycarbonate; PES: polyester; PET: polyethylene terephthalate; *before first change of medium; **after third change of medium.
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Fluids and Barriers of the CNS

ISSN: 2045-8118