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Figure 8 | Fluids and Barriers of the CNS

Figure 8

From: Comparative study of four immortalized human brain capillary endothelial cell lines, hCMEC/D3, hBMEC, TY10, and BB19, and optimization of culture conditions, for an in vitro blood–brain barrier model for drug permeability studies

Figure 8

Mean TEER (A) and P app (B) values for hBMEC cultured without growth factors in mono-cultures and contact co-cultures with SVG-A and HBPCT cells. A: Mean TEER values recorded real-time by the CellZscope system for hBMEC cells cultured without growth factors (w/o GF) in mono-cultures and co-cultures with SVG-A (black curve) or HBPCT cells (green curve). 24-Well tissue culture inserts from Greiner Bio-one® (transparent PET membrane, 3.0 μm pore size, 0.6 × 106 pores/cm2) were used (n = 3). Compared to co-cultures with SVG-A and HBPCT cells, hBMEC mono-cultures produced higher TEER values in the range of 40 Ωcm2 (blue curve). CCL values were in the acceptable range of 0.5-5.0 μF/cm2 (data not shown), indicating that hBMEC cells were confluent. The black arrow indicates the time when the permeability assay with Na-F was performed. B: Mean Papp values for Na-F through hBMEC cultured without growth factors through mono-cultures and co-cultures with SVG-A or HBPCT cells. Lowest Papp values for Na-F (5.11 ± 0.0487 × 10-6 cm/s) were obtained through hBMEC mono-cultures. Mean recoveries in all experiments were between 98% and 102% (n = 3).

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