Table 5 Materials for QTAP
Name | Company | Catalog# | Amount | Note |
|---|---|---|---|---|
Guanidine HCl | Wako | 070-01825 | 500 g | For denaturing buffer |
2NA (EDTA·2Na) | DOJINDO | 345-01865 | 500 g | For denaturing buffer |
Trizma® (Tris) base, primary standard and buffer | SIGMA | T1503-1KG | 1 kg | For several buffers |
Ammonium hydrogencarbonate | Wako | 017-02875 | 500 g | For LysC and ProteaseMax solution |
(±)-Dithiothreitol (DTT) | Wako | 049-08972 | 25 g | |
Iodoacetamide (IAA) | Wako | 093-02152 | 25 g | |
Chloroform | Wako | 038-02606 | 500 mL | |
Methanol | Wako | 137-01823 | 3 L | |
Urea | Wako | 211-01213 | 1 kg | |
ProteaseMax surfactant, trypsin enhancer | Promega | V2072 | 5 × 1 mg | |
Lysyl endopeptidase (LysC) | Wako | 129-02541 | 10 AU | |
Sequencing grade modified trypsin, frozen (TPCK-trypsin) | Promega | V5113 | 100 μg | |
SUMILON proteosave SS, 1.5 mL tube | SUMITOMO BAKELITE | MS-4215 M | 100/pk | Low protein binding tube |
SUMILON proteosave SS, 0.5 mL tube | SUMITOMO BAKELITE | MS-4205 M | 100/pk | Low protein binding tube |
Ultra plus tip 1–10 μL | bms | UP-0110 | 96/pk | Low protein binding tip |
Ultra plus tip 1–200 μL | bms | UP-2010 | 96/pk | Low protein binding tip |
Eyela cute mixer CM-1000 | TOKYO RIKAKIKAI | 188140 | Tube mixer | |
Bransonic ultrasonic cleaner 2510 J-DTH | Branson | 2510 J-DTH | Sonicator | |
High speed refrigerated micro centrifuge MX-160 | TOMY | Centrifuge | ||
TMA-30 | TOMY | Angle rotor | ||
TMS-21 | TOMY | Swing rotor | ||
Block incubator BI-525 | ASTEC | LysC digestion | ||
Incubator MIR-262 | SANYO | MIR-262 | Trypsin digestion | |
Formic acid (98.0%) | Wako | 066-00466 | 500 mL | Acidification |
Acetonitrile (LC/MS grade) | Wako | 018-19853 | 3 L | Mobile phase |
XBridge BEH130 C18 (1.0 × 100 mm, 3.5 μm) column | Waters | 186003561 | HPLC column | |
XBridge C18 Guard Cartridge (2.1 × 10 mm, 3.5 μm) | Waters | 186003059 | 2/pk | Guard column |
Sentry 2.1 mm guard holder | Waters | WAT097958 | Guard column holder | |
Agilent technologies 1200 series (HPLC system) | Agilent | HPLC | ||
API5000 | AB Sciex | QqQ mass spectrometer | ||
QTRAP5500 | AB Sciex | QqQ mass spectrometer | ||
96 well plates, 0.5 mL, polypropylene | Agilent | 5042-1386 | 10/pk | Sample plate for auto sampler (HPLC) |
Pre-slit well cap for 96 well PP plate non sterile, silicone | Thermo SCIENTIFIC | 276011 | 10/pk | Sample plate cap for auto sampler (HPLC) |
DC protein assay reagent A | BIO-RAD | 500-0113 | 250 mL | Protein quantification (Lowry method) |
DC protein assay reagent B | BIO-RAD | 500-0114 | 1 L | Protein quantification (Lowry method) |