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Figure 3 | Fluids and Barriers of the CNS

Figure 3

From: A study protocol for quantitative targeted absolute proteomics (QTAP) by LC-MS/MS: application for inter-strain differences in protein expression levels of transporters, receptors, claudin-5, and marker proteins at the blood–brain barrier in ddY, FVB, and C57BL/6J mice

Figure 3

Principle of peptide selection by selected/multiple reaction monitoring (SRM/MRM) mode of triple quadrupole mass spectrometry (QqQ MS). The selection of the targeted peptide by two mass filters (Q1 and Q3) results in the reduction of noise from the complex peptide sample. Each target peptide is monitored by four different SRM/MRM transitions, which consist of a parent ion (Q1) and four different daughter ions (Q3) for accurate and reliable quantification.

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