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Table 2 The fluorescence-based quantitative real-time PCR data for the reference gene and genes of interest in human choroid plexus.

From: Expression and functional activity of nucleoside transporters in human choroid plexus

 

Cq ± SD

(n = 5)

E-Cq ± SD (× 109)

18 S

13.97 ± 0.31

58123 ± 12920

hENT1

29.00 ± 0.52

5.23 ± 1.79

hENT2

28.08 ± 0.30

3.07 ± 0.62

hENT3

19.17 ± 0.59

1403 ± 494

hCNT1

> 40

-

hCNT2

> 40

-

hCNT3

27.67 ± 0.43

4.69 ± 1.35

  1. Values are presented as mean ± SD from 5 separate CP samples. For every sample the quantification or threshold cycle (Cq) value was calculated as an average from 2 replicates. The linear form of the Cq values was corrected for the reaction efficiency (E), and is presented as E-Cq. The Cq value was much lower for hENT3 than for other ENTs and CNTs, indicating that this nucleoside transporter was the most abundant at the transcript level.
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Fluids and Barriers of the CNS

ISSN: 2045-8118