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Table 2 The fluorescence-based quantitative real-time PCR data for the reference gene and genes of interest in human choroid plexus.

From: Expression and functional activity of nucleoside transporters in human choroid plexus

  Cq ± SD (n = 5) E-Cq ± SD (× 109)
18 S 13.97 ± 0.31 58123 ± 12920
hENT1 29.00 ± 0.52 5.23 ± 1.79
hENT2 28.08 ± 0.30 3.07 ± 0.62
hENT3 19.17 ± 0.59 1403 ± 494
hCNT1 > 40 -
hCNT2 > 40 -
hCNT3 27.67 ± 0.43 4.69 ± 1.35
  1. Values are presented as mean ± SD from 5 separate CP samples. For every sample the quantification or threshold cycle (Cq) value was calculated as an average from 2 replicates. The linear form of the Cq values was corrected for the reaction efficiency (E), and is presented as E-Cq. The Cq value was much lower for hENT3 than for other ENTs and CNTs, indicating that this nucleoside transporter was the most abundant at the transcript level.