Fluids and Barriers of the CNS

Table 4 Cytological rankings in hydrocephalic and control rats at P11 and P22/P23 as determined from immunohistochemical analysis.

From: Diffusion tensor imaging correlates with cytopathology in a rat model of neonatal hydrocephalus

		P11			P22/P23
		HCP (n = 3)	Control (n = 2)	Corrected p-value	HCP (n = 5)	Control (n = 5)	Corrected p-value
IC
	GFAP	2.67 ± 0.58	0.5 ± 0.00	NS	2.80 ± 0.28	0.25 ± 0.30	<0.05*
	Iba-1	2.67 ± 0.29	2.0 ± 0.00	NS	2.90 ± 0.22	0.90 ± 0.58	<0.05*
	LFB	n/d	n/d	n/d	1.69 ± 0.51	2.80 ± 0.21	<0.05*
EC
	GFAP	3.00 ± 0.00	0.69 ± 0.27	NS	3.00 ± 0.00	0.18 ± 0.14	<0.05*
	Iba-1	3.00 ± 0.00	1.5 ± 0.00	NS	3.00 ± 0.00	0.40 ± 0.39	<0.05*
	LFB	n/d	n/d	n/d	1.60 ± 1.04	2.15 ± 0.73	NS
FX
	GFAP	3.00 ± 0.00	0.88 ± 0.53	NS	2.75 ± 0.35	0.20 ± 0.33	<0.05*
	Iba-1	3.00 ± 0.00	0.5 ± 0.00	NS	2.85 ± 0.34	0.15 ± 0.14	<0.05*
	LFB	n/d	n/d	n/d	1.35 ± 0.60	2.20 ± 0.45	NS
CC
	GFAP	3.00 ± 0.00	0.75 ± 0.35	NS	n/d	n/d	n/d
	Iba-1	3.00 ± 0.00	0.75 ± 0.35	NS	n/d	n/d	n/d
	LFB	n/d	n/d	n/d	n/d	n/d	n/d

GFAP: glial fibrillary acidic protein for astrocytes, Iba-1: ionized calcium-binding adaptor molecule for microglia, LFB: luxol fast blue for myelin. Note. * significantly different (Wilcoxon two sample test); NS: not significant; n/d: not detectable; LFB stain too weak at P11 and CC too thin or missing at P22/P23. Brain regions as for figure 2.

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