Figure 7

Altered distribution of FGF2 in choroid plexus of rat brain after dehydration: FGF2 immunostaining in lateral ventricle choroid plexus tissues was evaluated in adult rats exposed to chronic water deprivation for 72 h. Anatomically, the choroidal villus consists of a single layer of cuboidal epithelial cells that circumferentially surrounds an inner vascular core and extracellular matrix. The apical membrane faces the 'outside' CSF compartment; see Smith et al. [40] for a delineated description of choroid structural features. Note the marked FGF2 immunostaining in plexus parenchymal epithelium from a dehydrated animal (A), particularly in the nuclei, cytoplasm and the apical (CSF-facing) membrane, and the lower concentration in basement membranes (B). Comparatively, normal animals show lower levels of intracellular FGF2 in the epithelium (C), but stronger staining associated with basement membranes (D). Tissues were immunostained with polyclonal antibodies against FGF2: Ab106 (A & C) and Ab773 (B & D).