Figure 3
A, B: Calcein-AM uptake assays show concentration-dependent inhibition by PSC833, MK571 and a cumulative effect of PSC833 and MK571. Control was set to 1. C, D: CMFDA uptake assays show strong inhibition by MK571 and also moderate inhibition by PSC833. Again an additive effect by combination of PSC833 and MK571 is observed. * = significantly different from control, P < 0.05, ** = significantly different from control, P < 0.01, *** = significantly different from control, P < 0.001. E, F: Uptake of [3H]bis-POM-PMEA into TR-CSFB cells (E) and Z310 cells (F), respectively, was enhanced by sulindac sulfide, MK571, dipyridamole, TC (taurocholate), DHEAS (Dehydroepiandrosterone) and PSC833 acting as inhibitors of Mrp4 mediated efflux. A, C and E show TR-CSFB cells, B, D and F show Z310 cells. * = significantly different from control, P < 0.05, ** = significantly different from control, P < 0.01, *** = significantly different from control, P < 0.001.