The subcommissural organ of the rat secretes Reissner's fiber glycoproteins and CSF-soluble proteins reaching the internal and external CSF compartments

Table 3 Immunoreactivity to AFRU and anti-P15 of proteins extracted from the rat subcommissural organ, Riessner's fiber and cisterna magna CSF

MW	SCO (PN30)		RF (PN30)		CSF (E18-PN7)
	AFRU	Anti-P15	AFRU	Anti-P15	AFRU	Anti-P15
630	+
450	+
390	+
320	+	+		+
200	+		+	+	+	+
180					+	+
164				+	+
145				+	+	+
120			+	+	+
80		+		+
63	*	*	*	*	+	*
50		*	*		+
45	*	*	+	+
37		+
32		+		+
25			*		+

MW, molecular weights in kilodaltons; SCO, subcommissural organ; RF, Reissner's fiber; AFRU, antibody against all glycoproteins of bovine RF; anti-P15, antibody specific for SCO-spondin; +, positive reaction consistently present in all samples; *, positive reaction only found in some samples. Horizontal box indicates 200 kDa compound consistently found in all samples. The eight AFRU-immunoreactive compounds detected in embryonic CSF were consistently found in all samples; only three of them react with anti-P15.

ISSN: 2045-8118