Skip to main content

Table 3 Immunoreactivity to AFRU and anti-P15 of proteins extracted from the rat subcommissural organ, Riessner's fiber and cisterna magna CSF

From: The subcommissural organ of the rat secretes Reissner's fiber glycoproteins and CSF-soluble proteins reaching the internal and external CSF compartments

MW SCO (PN30) RF (PN30) CSF (E18-PN7)
  AFRU Anti-P15 AFRU Anti-P15 AFRU Anti-P15
630 +      
450 +      
390 +      
320 + +   +   
200 +   + + + +
180      + +
164     + +  
145     + + +
120    + + +  
80   +   +   
63 * * * * + *
50   * *   +  
45 * * + +   
37   +     
32   +   +   
25    *   +  
  1. MW, molecular weights in kilodaltons; SCO, subcommissural organ; RF, Reissner's fiber; AFRU, antibody against all glycoproteins of bovine RF; anti-P15, antibody specific for SCO-spondin; +, positive reaction consistently present in all samples; *, positive reaction only found in some samples. Horizontal box indicates 200 kDa compound consistently found in all samples. The eight AFRU-immunoreactive compounds detected in embryonic CSF were consistently found in all samples; only three of them react with anti-P15.