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Table 2 Comparative analysis of AFRU-immunoreactive proteins extracted from the rat subcommissural organ, Reissner's fiber and from the cisterna magna CSF

From: The subcommissural organ of the rat secretes Reissner's fiber glycoproteins and CSF-soluble proteins reaching the internal and external CSF compartments

MW SCO (PN30) RF (PN30) CSF (E18-PN7) CSF (PN30)
  AFRU ConA WGA AFRU AFRU AFRU
630 + + -    
450 + + +    
390 + + -    
320 + + +    
200 + + + + + +
180      +  
164      +  
145      +  
120     + +  
63 *    * + +
50     * + +
45 *    +   
25     * + +
  1. MW, molecular weights in kilodaltons; SCO, subcommissural organ; RF, Reissner's fiber; AFRU, antibody against all glycoproteins of bovine RF; Con A, concanavalin A (affinity = mannose, glucose); WGA, wheat germ agglutinin (affinity = glucosamine, sialic acid); +, positive reaction consistently present in all samples; *, positive reaction only found in some samples. Two upper boxes indicate putative precursor forms only present in SCO. Lower box indicates the only compound consistently found in SCO, RF and CSF (processed form).