Figure 1
Expression of Fgf2 and p-H3 in the mouse and human CPe. (A) IV ventricle (IV) choroid plexus epithelium (CPe) in a sagittal section of E12.5 mouse embryo brain; positive cytoplasmic staining for Fgf2 protein is observed in the CPe and less strongly in the CP mesenchyme (CPm). (B) Negative control for (A). (C) Human IV ventricle CPe at 8 weeks of gestation: strong Fgf2 labelling is present in the CPe. (D) Human lateral ventricle CPe at 8 weeks of gestation; Fgf2 staining is mainly cytoplasmic. (E) Negative control for (D). (F) P-H3 staining of coronal section of E12.5 mouse embryo whole brain, including the choroid plexuses of the lateral ventricles. Neuroepithelial cells of the ventricular zone are frequently labelled with p-H3 antibody whereas p-H3 labelling in the CPs is rare. (G) Black arrow indicates p-H3 labelled cells in the IV ventricle CP stalk; red arrow indicates a p-H3 labelled cell in the CPm. Scale bars are 50 μm in A-B; 15 μm in C-D; 500 μm in F and 200 μm in G. CP = choroid plexus, CPe = choroid plexus epithelium, CPm = choroid plexus mesenchyme, L = lateral ventricle, N = neuroepithelium, VZ = ventricular zone, III = third ventricle, IV = fourth ventricle.