Volume 3 Supplement 1
Long-lasting hydrocephalus in hyh mutant mice: gain and loss of a brain surviving hydrocephalus
© Rodríguez et al; licensee BioMed Central Ltd. 2006
Published: 21 December 2006
A population (30%) of hydrocephalic hyh mutant mice develop a slowly progressive hydrocephalus and survive for periods ranging between 2 months and 2 years. Certain characteristics of these mice, such as time of onset of hydrocephalus, type of abnormality of CSF dynamics, clinical evolution and survival/death rate, resemble several types of human congenital hydrocephalus. They represent an exceptional animal model to investigate neuropathological and physiopathological aspects of a brain "adapting" to a virtually life-lasting hydrocephalus
Materials and methods
(i) The clinical evolution of more than 3,000 hyh mice, (ii) certain cellular and molecular aspects involved in the pathogenesis of hydrocephalus of several hundreds of embryos and postnatal hyh specimens and, (iii) the neuropathology of more than one hundred hydrocephalic mice with arrested hydrocephalus have been investigated with a large series of techniques.
The events occurring in the brain of aging hydrocephalic mice may be regarded as loss and gain. Loss: Neuroepithelium/ependyma denudation is a severe loss, since it leads to (i) Sylvius aqueduct obliteration and severe hydrocephalus; (ii) abnormal development of certain populations of cerebral neurons resulting in a permanent neurological impairment. Abnormalities in the subcommissural organ contribute to the development of hydrocephalus and to changes in the protein composition of CSF. Severe alterations of hypothalamus lead to neuroendocrine deficiencies. Gain: (i) A subpopulation of astrocytes responds to denudation by repairing the denuded areas forming an ependymal-like new barrier. (ii) Once severe hydrocephalus has been turned on, two ependymal populations located in the aqueduct and third ventricle start to proliferate allowing the large expansion of these cavities. This ependymogenesis continues for several weeks after birth. (iii) All mice surviving hydrocephalus developed spontaneous ventriculostomies.
(i) Essential events of the hydrocephalic phenomenon occur at a rather well defined temporal and spatial pattern in which a program of sequential events may be envisaged. (ii) None of the pathological events should be regarded as the result of mechanical phenomena. Rather, abnormalities occurring at molecular and cellular levels and leading to ependymal denudation and abnormal neurogenesis, precede the onset of hydrocephalus. (iii) Postnatal ependymogenesis and spontaneous ventriculostomies are essential for the hydrocephalic mice to live a long life.
Fondecyt 1030265, Chile to EMR; FIS PI 030756, Red CIEN (C/0306), SAS, Spain to JMP-F.
This article is published under license to BioMed Central Ltd.