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Table 1 The permeability values (Pe - x 10-3cm/min) of laminin-coated inserts without cells (w/o cells), laminin coated inserts with 8d-old CPEC monolayer (with cells control) and laminin coated inserts with 8d-old CPEC monolayers which were pre-treated to reduce adenosine metabolism (with cells inhibition). [14C] adenosine was used as a test molecule and [3H] mannitol as an inert reference of the paracellular diffusion. From these values the permeability of CPEC monolayers (Pcells) was calculated and then permeability for mannitol subtracted from the permeability for adenosine to estimate permeability of cellular monolayers for [14C] adenosine. All values are mean+/- SEM from 3–4 different inserts, which were produced from at least two animals.

From: Uneven distribution of nucleoside transporters and intracellular enzymatic degradation prevent transport of intact [14C] adenosine across the sheep choroid plexus epithelium as a monolayer in primary culture

  Upper chamber as the donor Lower chamber as the donor
  Adenosine Mannitol Adenosine Mannitol
W/o cells 3.26 ± 0.76 3.40 ± 0.068 3.09 ± 0.41 3.32 ± 0.22
With cells control 0.64 ± 0.17 0.35 ± 0.11 0.73 ± 0.21 0.55 ± 0.08
With cells inhibition 1.50 ± 0.15 0.39 ± 0.10 1.35 ± 0.16 0.44 ± 0.08
Pecells control 0.79 ± 0.05 0.39 ± 0.06 0.95 ± 0.11 0.64 ± 0.07
Pe ado -Pe mannitol control 0.40 ± 0.03 0.31 ± 0.04
Pecells inhibition 2.78 ± 0.14 0.44 ± 0.11 2.39 ± 0.17 0.67 ± 0.09
Pe ado -Pe mannitol inhibition 2.34 ± 0.17 1.72 ± 0.14