Localization of inflammatory cell infiltrates in the brain of mice suffering from EAE are enriched in close vicinity to CVOs. During EAE, CD45+ cellular infiltrates in the brain were found to be enriched in close vicinity to CVOs. The top panel shows the SFO with perivascular CD45+ cells present within the CVO. Massive CD45+ infiltrates are prominent within the ventricular walls and the brain parenchyma outside but in close vicinity to SFO. The bottom panel shows the AP with perivascular and parenchymal CD45+ cells present within this CVO. Many CD45+ cells have accumulated at the border between the AP and the 4th ventricle. These pictures are representative for the finding that CD45+ inflammatory cell infiltrates in the brain were found to be enriched in number and size in close vicinity to CVOs during EAE. Immunoperoxidase, hematoxylin counterstain. Bar = 100 μm. This result was observed in at least 10 individual stainings.