Fig. 6
Species selectivity transport of Tf and TfR-targeting antibodies across the BBB. The in vitro apparent permeability coefficient (PAPP) assessed by MRM of holo-transferrin (Tf) in A the mouse mBEC and B human iBEC BBB models (mean ± SD). C Comparison of the in vitro apparent permeability coefficient (PAPP) for TfR targeting test antibodies J05-Fc, 8D3 (1.2 nM) in the presence or absence of 2 mg/ml of Tf in the luminal chamber in the mouse mBEC model (mean ± SD). A20.1 (targeting C. Difficile Toxin A) was used as a negative transcytosis control antibody (mean ± SD). LLOQ (Lower limit of quantification); ULOQ (Upper limit of quantification). Results are from two independent differentiations