Fig. 5
Functional receptor mediated transcytosis in mBECs. A RNASeq heatmaps depicting log2 transformed transcript abundances of expression of receptors involved in mediating receptor-mediated transcytosis (RMT) in mBEC, bEnd.3 and pmBEC. Green: low expression; Red: high expression. B Representative Wes blots confirming protein expression of key RMT receptors: TfRC, TMEM30A, LRP1, INSR; β-ACTIN was used as loading control. C, D Flow cytometry analysis assessing TfR1 and TMEM30A expression in mBECs. Red = unstained control. The in vitro apparent permeability coefficient (PAPP) was assessed by MRM for TfR targeting antibodies J05-Fc, 8D3 and 8D3-130 as well as the species cross-reactive FC5-Fc (targeting TMEM30A) and IGF1R5-Fc (targeting IGF1R) in E the mouse mBEC and F and human iBEC BBB models. A20.1 (targeting C. Difficile Toxin A) was used as a negative transcytosis control antibody (mean ± SD). LLOQ (Lower limit of quantification); ULOQ (Upper limit of quantification). Results are from two independent differentiations