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Table 1 Blue-light-induced cytotoxicity depends on the illuminance and exposure time

From: Blue light exposure collapses the inner blood-retinal barrier by accelerating endothelial CLDN5 degradation through the disturbance of GNAZ and the activation of ADAM17

Exposure time (h)

Cell density (counts/field)

SDH activity (% of control)

80 lx

160 lx

240 lx

80 lx

160 lx

240 lx

bEnd.3 cell

  

 0

284.8 ± 6.4

100.2 ± 0.2

 6

288.3 ± 14.3

283.9 ± 10.3

295.6 ± 4.8

97.6 ± 1.3

96.7 ± 1.9

82.8 ± 2.8***

 12

288.0 ± 21.2

277.9 ± 9.4

222.3 ± 6.3***

94.5 ± 4.4

84.2 ± 1.1**

69.8 ± 2.4***

 24

273.0 ± 9.0

232.8 ± 21.7*

195.4 ± 6.2***

79.8 ± 3.5***

58.5 ± 8.7***

32.2 ± 14.8***

HREC

  

 0

243.1 ± 10.7

100.0 ± 0.0

 6

236.3 ± 16.6

236.8 ± 9.2

214.9 ± 5.8

98.9 ± 4.8

100.6 ± 3.7

97.8 ± 3.6

 12

234.9 ± 14.5

231.5 ± 9.9

195.3 ± 11.5*

100.4 ± 5.5

94.8 ± 3.8

81.9 ± 5.1**

 24

223.5 ± 14.1

192.5 ± 8.3*

175.5 ± 14.8**

87.5 ± 3.3**

66.8 ± 7.5**

57.1 ± 12.2**

  1. Cell viability was determined by cell density (crystal violet assay) and succinate dehydrogenase (SDH) activity (MTT assay)
  2. Blue light reduced SDH activity and cell density at 240 lx exposure for > 6 h (bEnd.3) and > 12 h (HREC). In addition, 24 h blue light exposure > 80 lx significantly reduced SDH activity in both endothelial cell lines. Therefore, exposure to 240 lx illuminance and a 24 h exposure period were not favorable for further experiments. The endothelial cells were less viable but were still alive after exposure to 160 lx blue light for 12 h. The conclusions drawn from the in vitro data were mostly dependent on the 160 lx. This illuminance is frequently achievable in 3C devices and is feasible in terms of reading distance
  3. *p < 0.05, **p < 0.01, ***p < 0.001, indicates statistical difference from the control
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Fluids and Barriers of the CNS

ISSN: 2045-8118